镜聚焦后的边缘光线(marginal rays)与近轴光线(paraxial rays)经过不同的生物组织，折射的程度也不相同。最后，当样本浸在液体里时，因为样本会流动不易被观察，
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边缘光线集点 marginal focus
边缘光线高度 RH ; Marginal Ray Height
只画边缘光线和主光线 Marginal and Chief Only
边缘光线角 RA
在充足的光线下，树林边缘的树木呈现出一种暗棕色的绿色——因为已经到八月底了。
The trees on the wood-edge were a darkish, brownish green in the full light—for it was the end of August.
这个新镜头，底部比顶部更薄，通过切换镜头底部边缘的发光二极管的开和关，控制进入到观众的眼睛的光线。
The new lens, which is thinner at the bottom than at the top, steers light to a viewer's eyes by switching light-emitting diodes along its bottom edge on and off.
经由透明的玻璃边缘，光线穿透到玻璃幕墙的内层表面，这样白天自然光就能让教堂闪亮，而晚上的时候教堂内发出的光也能让它的外表闪闪发亮。
Passing through clear glass margins, light strikes the interior surface of these veils causing them to glow with natural light during the day and light from the sanctuary8 at night.
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