无明确的逆转录病毒载体质粒的致突变性的证据。

There is no evidence of the mutagenicity of the plasmid.

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重组人胰岛素基因逆转录病毒载体质粒应用于活体动物是安全的。

The application to rats of recombinant human insulin gene retroviral vector plasmid mutated in three place with physiological regulatory element constructed by us was safe.

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实验结果表明，以TK基因构建的重组载体质粒可用于外源基因的重组表达研究。

The result showed that the transfer vector plasmid constructed with FPVTK genes can be used to express foreign gene in FPV recombinant.

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The sources are: one,the plasmid vector that we've picked, and the second is these genes that for some reason we're interested in.

第一个是我们选择的质粒载体,第二个是我们感兴趣的基因

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The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

Then we're going to take these plasmid vectors and we're going to somehow put them in contact with cells in such a way that the cells ingest the DNA and they use it.

然后要使这些质粒载体,以一种特别的方式与细胞接触,让细胞吞入质粒载体并使之表达

耶鲁公开课 - 生物医学工程探索课程节选