RT-PCR for the cartilage specific mRNA,typeⅡcollagen,aggrecan,SOX-9 and the dedifferention marker of type X collagen were analyzed after 3 weeks to show the state of chondrogenic differentiation.
分别于诱导的第14d和21d,用RT-PCR法检测3组细胞Ⅱ型胶原,X型胶原,aggrecan,SOX-9 mRNA的表达情况,分析各个组成软骨分化的情况。
参考来源 - 不同培养方式诱导脂肪干细胞成组织工程软骨的实验性研究Our research showed:different concentration of TGF-β_1 had different effects in chondrogenesis,10μg/L was the best concentration for chondrogenesis.
研究表明:不同浓度的TGF-β_1对于促进MSCs的成软骨分化作用效果不同,10μg/L为促进软骨分化的最佳浓度。
参考来源 - 人骨髓间充质干细胞体外培养及成软骨诱导研究ObjectiveTo study the cartilage differentiation of mouse bone marrow mesenchymal stem cells(BMSCs) induced by cartilage-derived morphogenetic protein-2 in vitro. MethodsThe BMSCs were isolated from mouse bone marrow and cultured in vitro.
目的研究软骨源性形态发生蛋白-2(CDMP-2)对大鼠骨髓间充质干细胞(BMSCs)向软骨分化的作用。
参考来源 - 软骨源性形态发生蛋白·2,447,543篇论文数据，部分数据来源于NoteExpress
目的综述近年骨髓基质干细胞成软骨分化的研究进展。
Objective To review the recent research progress about the chondrogenic ability of bone marrow-derived mesenchymal stem cells.
在细胞表面标志上，年轻与成年兔形成层及软骨组织表现较多具软骨分化潜力之细胞标志。
For the mesenchymal stem cell marker profiles, cambium layer and cartilage layer all expressed higher chondroprogenitor related marker profiles.
目的研究软骨分化形成蛋白- 2 (CDMP - 2)对小鼠骨髓基质干细胞软骨分化的作用。
Objective To study the cartilage differentiation of mouse mesenchymal stem cells (MSCs) induced by cartilage-derived morphogenetic proteins-2 (CDMP-2) in vitro.
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