方法：将基因内翻译起始序列合成后克隆到上游含有起始密码和无起始密码的报告质粒中，研究表达水平的差异。
Methods:One of the three internal translation initiation region(TIR) was chemically synthesized and cloned into the report plasmid, and the expression of the report gene was studied.
随后在indent _ pat中构建一个新的正则表达式，精确匹配任何非空行的起始处的相同序列的空白(即拖尾' \ S ')。
It then builds a new regular expression in indent_pat that matches exactly the same sequence of whitespace at the start of any non-empty line (hence the trailing '\ s').
当聚合酶将一个核苷酸加在新互补链的起始引子之后，或接合酶认定某段核苷酸链与原始模版配对，就可利用这些反应来得出原始模版的序列。
As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template's sequence is revealed.
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