综述了近年来有关双歧杆菌质粒分离、载体构建、基因组测序以及在基因治疗上的应用等研究进展。

This paper reviews recent relevant bifidobacterium plasmid isolation, vector, genome sequencing and gene therapy in the application of research progress.

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采用含HBV全基因组的重组质粒DNA对355份肝炎患者或正常人群的血清样本进行检测，评价该系统的效果。

This system was evaluated using plasmid DNA with complete HBV genome and 355 sera from hepatitis patients and normal persons.

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本文报道了利用细菌的全基因组信息和质粒拯救法的原理建立的一种克隆细菌基因组大片段的方法。

To develop a new method to cline large DNA fragment by combining whole genome sequence information and the principle of plasmid rescue.

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