Objective To analyze the differences of Glycosyltransferases gene expression profile in different human tumor cells with cDNA microarrays.
目的 研究采用低密度基因芯片对人不同肿瘤细胞中的糖基转移酶基因家族进行表达谱分析。
参考来源 - 不同肿瘤细胞株三种糖基转移酶基因家族低密度表达谱基因芯片的初步研究Tissue expression pattern analysis found that AaH6H expresses in all the tested tissues including roots, stems and leaves and indicated that AaH6H is a constitutive-expression gene, which was the first reported tissue-independent H6H gene compared to other known H6H genes.
组织特异性表达谱分析发现,AaH6H基因在根、茎、叶三种组织中都有表达,表明AaH6H是一种组成型表达基因,这在含托品烷生物碱植物中还是首次发现。
参考来源 - 三分三中托品烷生物碱合成途径中关键酶基因的克隆及特征分析·2,447,543篇论文数据，部分数据来源于NoteExpress
经表达谱分析发现，NECL2在多组织中广泛表达，如脑、心、肝、肺、骨骼肌等。
NECL2 is widely expressed in multiple tissues, such as brain heart, liver lung and skeletal muscle by using Northern Blot.
通过生物信息学est分析，以及组织表达谱分析，证实它在正常组织中广谱表达，具有肿瘤组织表达特异性。
Using EST and tissue expression pattern analysis, this gene is proved to be widely expressed in normal tissues while specifically expressed in tumor tissues.
目前，该技术已用于基因表达谱分析、新基因发现、基因突变、基因多态性分析、药物筛选和基因测序及疾病的诊断。
By the mean time, the technique have reported in the gene expression analysis, new gene discovery, gene mutation detection, SNP analysis, DNA re-sequence and diagnosis.
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