...外周血淋巴细胞膜蛋白,经SDS-PAGE检测后,湿转印法转印至NC膜,分别利用纯化的重组tH蛋白和PPRV进行病毒铺覆蛋白结合试验（VOPBA）,对受体进行鉴定。【结果】成功克隆了1 653bp的PPRVtH基因,构建其重组酵母表达质粒pPIC9K-tH,诱导表达后获得了60ku目的蛋白。
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蛋白结合碘试验 [临床] protein-bound iodine test
白蛋白钴结合试验 albumin cobalt binding test
蛋白质结合碘试验系统 Protein-bound iodine test system
2007年的试验结果令人眼界大开：染色体上藏着许多以前不为人知的位点，那里结合着各种各样的蛋白——可能是基因调控和表观遗传效应的温床。
The 2007 pilot results were eye-opening: Chromosomes harbored many previously unsuspected sites where various proteins bound—possible hotbeds of gene regulation or epigenetic effects.
表达产物用亲和层析一步法进行纯化，然后用凝胶阻滞试验观察重组蛋白结合质粒的能力。
The expressed protein was purified by one-step affinity chromatography, and its ability of binding plasmid was investigated via gel retardation experiments.
采用饲养试验、消化代谢试验和配种试验相结合的方法，测定了成年种公羊能量和蛋白质代谢的基础数据。
The basic data on the metabolism and utilization of energy and protein of the breeding RAMS were obtained by feeding experiments, digestion and metabolism trials and breeding tests.
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