蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
实验采用原位杂交，NADPH - NBT组织化学，RNA斑点印迹及蛋白质斑点印迹技术。
The experiment was performed by using in situ hybridization, NADPH NBT histochemistry, RNA dot blot and protein dot blot techniques.
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