将除琼脂以外的各成分溶解于蒸馏水内,加入15%氢氧化钠溶液约2mL,校正pH至7.2~7.4。加入琼脂,加热煮沸,使琼脂溶化。分装烧瓶,122℃高压灭菌15min。 注:此培养基可供一般细菌培养使用,可倾注平板或制成斜面。如用于菌落计数,琼脂量为1.5%;如作成平板或斜面,则应为2%。
营养琼脂(nutrient agar, NA):取 Difco Bacto-nutrient agar 23 g 加入 1,000 ml 蒸馏水中...
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... nutrient agar medium 营养琼脂 Developed Nutrient Agar 改良马丁琼脂培养基 semisolid nutrient agar 半固体营养琼 ...
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...通琼脂斜面培养基(化),品质有保证的培养基产品,BRP0434,MiddleBrook7H11琼脂用于分枝杆菌的分离培养250克P0443,营养琼脂(NA),品质有保证的培养基产品,BR...
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结论,该植物复方精油熏蒸法,对营养琼脂平板表面上细菌具有一定杀灭作用。
Conclusion: the plant compound essential oil fumigation has certain killing effect on the bacteria on nutrient AGAR plate.
方法自然沉降法,利用重力作用使空气中的微生物粒子自然沉降在营养琼脂培养基上。
METHODS Natural sedimentation, where microbial particles in the air were settled by gravity on nutrient AGAR media before and after disinfection.
结论以营养琼脂、胎牛血清、抗生素按一定比例制成固体支架,可以用来培养动物软组织细胞。
Conclusion The scaffold made of AGAR, embryo cattle serum and antibiotics could be used to culture parenchyma cells of animals.
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