用免疫荧光组织化学法检测脑缺血再灌注后早期大鼠大脑梗死中心区、梗塞周边区和缺血对侧NG2和O4阳性细胞数量。

Then to determinate NG2 of O4 positive cells in the ischemic core, ischemic penumbra and contralateral area of rats at each time-point after reperfusion by immunofluorescence histochemistry.

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方法SD大鼠单次全脑照射后用免疫荧光组织化学法分别检测早期大脑皮质和海马ca 1区NG2和O4阳性细胞数量。

Methods to determine, by immunofluorescence histochemical method, NG2 and O4-positive cells in the cortex and hippocampal CA1 area of SD rats early after single dose whole-brain irradiation.

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结果：免疫荧光和免疫组织化学法均表明成纤维细胞生长因子18在MDPC - 23细胞浆呈阳性表达。

RESULTS: Fibroblast growth factor 18 was expressed in cytoplasm of MDPC-23 cell by both immunohistochemical and immunofluorescent stainings.

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