已分化的细胞经过诱导后失去其特有的结构和功能而转变成未分化细胞的过程叫脱分化（dedifferentiation）。 脱分化与再分化是植物组织培养的重要过程。
3、脱分化(dedifferentiation)：植物已经分化的细胞在切割损伤或在适宜的培养基上诱导形成失去分化状态的，结构均一的愈伤组织或细胞团的过程。
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It was recommended that microspores first be cultured at 35℃or 32℃less than 24 hours was advantage of the dedifferentiation of microspore.
35℃和32℃高温预处理不超过24h对小孢子的脱分化是有利的。
参考来源 - 辣椒花药小孢子培养及其胚状体发生机理研究The current transformation methods mainly depend on the culture approach of dedifferentiation and re-differentiation of plant cells to transfer foreign genes and obtain transgenic plants.
目前的转基因技术主要依靠植物细胞脱分化和再分化的组织培养方法,实现外源基因的转移,获得转基因植株。
参考来源 - 植物生长点转化法的初步建立·2,447,543篇论文数据，部分数据来源于NoteExpress
脱分化途径产生的 生姜愈伤组织分化频率低，不利于 生姜组织培养。
The differentiation rate of callus produced by the pathway of de-differentiation was low, which was disadvantage to tissue culture of Zingiber offcinai Roscoe.
作者在文中还讨论了烟草叶细胞脱分化和再分化与植物激素、蔗糖和培养材料的关系。
The authors still discussed the relation of dedifferentiation and redifferentiation of leaf cells of tobacco with plant hormon.
用组织培养的方法使甜瓜子叶脱分化形成愈伤组织，继而在分化培养基上形成不定芽。
The method of tissue culture is used to induce the cotyledons of cucumis melon to become callus and then adventitious buds.
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