用薄层聚丙烯酰胺凝胶等电聚焦电泳测得该酶的等电点为3.85和4.35；
Its isoelectric points are 3.85 and 4.35, determined by iso-eletric focusing electrophoresis.
方法：采集33例正常人，26例牙周炎患者全唾液，运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
Methods: Saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.
定量后，分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.
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