免疫脾细胞与骨髓瘤细胞融合后接种12块96孔板。
Confluenced immune spleen cells were inoculated in 12 plates with 96 Wells each.
结论骨巨细胞瘤中的多核巨细胞可能是由单核基质细胞融合而成,ADAM12基因参与了这一融合过程。
Conclusion Multinucleated giant cells probably originated from the fusion of mononuclear stromal cells and ADAM12 may participate in the fusion process.
将纯化之球蛋白片段 免疫 BALB/c 小鼠后, 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1 进行细胞融合。
The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.
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