为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。
Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.
说明血液灌注液比晶体液的保护效果明显。可能是血液在心肌缺血状态提供氧基质，保证细胞代谢和维持内环境的稳定。
The possible mechanism is that blood can provide energy substrate for ischemic myocardium and maintain the cellular metabolism and homeostasis.
细胞外液包含营养物质和维持细胞生存所需要的其他成分。
The extracellular fluid contains the nutrients and other constitutions necessary for maintenance of cellular life.
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