背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。

BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.

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目的：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。

AIM: the key of the human embryonic stem cell culture is to guarantee the totipotency and inhibit spontaneous differentiation.

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动物细胞在胚胎发育的较早阶段便失去了其全能性。

Animal cells lose their totipotency at an early stage in embryonic development.

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