病理染色图象分析测定左室腔周长和面积、单位面积内心肌细胞核数、单位面积内胶原的含量；

Pathological staining and image analysis were used to determine the perimeter and the area of left ventricular cavity, and myocardial nuclei number and collagen content per unit area;

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在分离的LV上以相同方向、均匀的切片，使用无偏倚的立体学方法，评估心肌细胞核总数、每个心肌细胞的细胞核数。

Isotropic, uniformly random sections were cut from theisolated LV, and the total number of myocyte nuclei and the average number of nuclei per myocyte were estimated using unbiased stereology.

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将其分为老年鼠对照组和药物组(85周龄)、青年鼠对照组(12周龄)，以肝细胞核d na单链断裂数和全血细胞非程序dna合成(uds)为观察指标。

The young (12 weeks) and old (85 weeks) rats which were divided into three groups, were used to measure the DNA single chain break of liver nuclei and unscheduled DNA synthesis (UDS) of blood cells.

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