无缝钢管的穿孔过程是一个热加工过程，顶头的温度分布对于其寿命至关重要。
The piercing process of seamless steel tube is a hot working process, the distribution of plug temperature is very important to its service life.
温度和缓冲液中的血清成分对电穿孔效率影响不大。
The operation temperature and the concentration of serum in buffer have no effect on the transfection efficiencies.
分别改变脉冲电压和环境温度，研究了酵母细胞的电穿孔情况。
Separately changes the pulse voltage and temperature in the study of yeast cells by electroporation.
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