二抗的最佳稀释度根据实际应用来确定。最终稀释度为1：500时，对于大多数的免疫荧光分析都能得出可取的结果。
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most immunofluorescent applications.
二抗的最佳稀释度根据实际应用来确定。最终稀释度为1：500时，对于大多数的流式细胞分析都能得出可取的结果。
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.
在家里直接加一小撮盐也没问题，可在酒吧我们需要更加精确，因此我们将使用稀释盐水：将20克食盐融于80毫升水（稀释度20%）。
At home you can get away with adding a pinch. At the bar we have to be more precise, so we use a saline solution: 20 grams of salt in 80 milliliters of water (20 per cent solution).
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