用差速离心法提取心肌线粒体，考马斯亮蓝法测定蛋白含量。
Myocardial mitochondria was extracted by differential centrifugation, and protein level was measured by Coomassie brilliant blue method.
还用台盼蓝拒染法确定细胞活力以及培养AML细胞离心涂片细胞化学染色观察细胞形态。
The viability of cultured AML cells was also evaluated by Trypan blue exclusion. Cytospin specimens of cultured AML cells were studied by cytochemical staining.
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