中间有一个碱基错配的 mis-match
一种修复DNA分子中错配碱基的机理。
A mechanism for the repair of mismatched bases in a DNA molecule.
目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。
Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.
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