石蜡包埋表皮组织中β-连环蛋白基因扩增技术的建立_stmopen 关键词】: 聚合酶链反应 石蜡包埋组织 β-连环蛋白 [gap=852]Keywords】: polymerase chain reaction； paraffin-embedded tissue； β-catenin；
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甲醛固定石蜡包埋组织 FFPET ; formalin-fixed paraffin-embedded tissues
固定石蜡包埋组织 FFPET
石蜡包埋组织标本 paraffin-embedded tissue sample
石蜡包埋的组织 FFPET
Background and objective Formalin-fixed and paraffin-embedded tissue (FPE) with long term follow-up and clinicopathological data are extensively available and easily accessible. They represent an extensive source of genetic materials and proteins to be investigated for clinical usage.
背景与目的甲醛固定石蜡包埋组织(FPE)包含大量临床病理信息,同时还含有大量的基因和蛋白物质可用来研究临床意义。
参考来源 - 从石蜡包埋肺癌组织提取RNA检测PML基因转录的研究Objective: To take degraded DNAremains in the aged bones、teeth、formalin fixed and paraff in embedded tissues (FFPET) as the research subject, and adopts and compares different techniques and methods to find a suitable method for degraded sample extraction.
目的:本论文以陈旧骨骼、牙齿及福尔马林固定石蜡包埋组织中残留的少量降解的核酸分子作为研究对象,利用多种技术方法,比较并建立适合降解检材的提取技术方法。
参考来源 - 疑难降解检材DNA检验方法研究·2,447,543篇论文数据，部分数据来源于NoteExpress
从石蜡包埋组织中提取DNA，是进一步研究抑癌基因的基础。
It is the foundation to further research anti-oncogene to extract DNA from paraffin embed constitution.
结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR.
结论：合适的福尔·马林固定石蜡包埋组织dna提取方法可应用PCR进行DNA水平的相应的研究。
Conclusion: Appropriate DNA extract methods for formalin fixed and paraffin embedded tissues may benifit PCR.
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