生物素化是指将生物素共价连接到蛋白质、核酸或其它分子上的过程。
2．血清样本蛋白标记 2．1．血清样本蛋白的 生物素化 （ Biotinylation ） （1）lmg 生物素化 试剂（BiotinylationReagent） 溶解于O．1ml的N，N一二甲基甲酰胺 DMF fN’NDimethylformamide），制成浓度...
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主题：生物素 | 医源世界 关键词】 生物素化；HCV抗原；标记率；纯度 [gap=882]Key words】 biotin alization;HCV antigen;labeling rate;purity
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2. 5 phage antibody clones were obtained against biotinylated melamine after 3 times of enriched procedure using the method in the second part. The ELISA and the competitive inhibitio ELISA results proved they had very good specificity.
2.利用第二部分方法,对生物素化的三聚氰胺进行3轮“吸附—洗脱—扩增”筛选,得到5株噬菌体阳性克隆,ELISA和竞争抑制ELISA结果也证明其具有较好特异性。
参考来源 - 盐酸克伦特罗单链抗体（scFv）的筛选与表达Measure1. TUNEL positive cell testAdd the slice with drops of TDT and digoxin-marked dUTP reaction fluid, incubated at 37℃;then add biotinylation anti-digoxin antibody,incubated at 37℃for 30min,washed by 0.01MTBS(pH7.5) 3min for 3 times;stain with DAB,restain with hematoxylin,dehydrate,transparent and mount.
二、检测指标1、TUNEL阳性细胞检测切片滴加脱氧核糖核酸转移酶(TDT)和地高辛标记的dUTP反应液,37℃孵育120min;滴加生物素化抗地高辛抗体,37℃孵育30min,各步骤用0.01MTBS(pH 7.5)洗涤3min×3次;DAB显色,苏木素复染,脱水、透明、封片。
参考来源 - TLR4对大鼠脑出血后炎症反应和神经细胞凋亡的作用研究·2,447,543篇论文数据，部分数据来源于NoteExpress
抗体生物素化后活性保持较好，生物素化效率较高。
The biological activity and the biotinylation efficiency of antibody keeps well after biotinylation.
本产品已经过优化，可应用于检测生物素化的蛋白标记。
This product has been optimized to detect biotinylated protein markers.
目的：探讨生物素化葡聚糖胺对皮质脊髓束示踪的应用价值。
Objective: to explore the applicative value of biotinylated dextran amine to anterograde tracing of the corticospinal tract in rat.
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