点突变指只有一个碱基对发生改变。广义点突变可以是碱基替换,单碱基插入或碱基缺失;狭义点突变也称作单碱基替换(base substitution)。碱基替换又分为转换(transitions)和颠换(transversions)两类。点突变具有很高的回复突变率。
... mutation rate突变率;变异概率;突变速率 point mutation点突变 genetic mutation基因突变 ...
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定点突变 site directed mutagenesis ; site-directed mutation ; oligonucleotide-directed mutagenesis ; Sequencing
基因点突变 [遗] point mutation ; gene site-directed mutation
终点突变 end point mutation
零点突变 zero point mutation
起点突变 zero point mutation
多点突变 Multiple mutation ; multipoint mutation
密码子点突变 codon of mutation site
点突变检测 TILLING
Objective:To establish a sensitive and specific test for JAK2V617F point mutation.
目的:建立敏感特异的JAK2V617F点突变的临床检测方法。
参考来源 - 等位基因特异性Conclusion 1 . p16 gene mutation is early event in gastric carcinogenesis , It helps early diagnosis of human gastric carcinoma.
结论:1.p16基因点突变是胃癌发生的早期事件,对胃癌早期诊断有一定帮助。
参考来源 - 不同时期胃癌中p16基因缺失和突变的研究Many thennostable enzymes have been improved intrisincally by site-directed mutagenesis,but increasing the thermostability of the enzymes in the presence of substrate by such mutation method is rarely been reported.
虽然底物对酶的热稳定作用是被报道得较多的,但通过点突变提高底物对蛋白质的热稳定作用却很少被报道。
参考来源 - 11家族木聚糖酶的热稳定性与结构特征的关联研究RibAH mutation gene fragment was obtained by overlap PCR, and was transformed to B. subtilis 24 Y1. B. subtilis 24 Y3 was gained in which ribAH contain two site mutation by homologous recombination.
通过重叠PCR扩增了一段带有点突变的ribAH基因,经转化B. subtilis 24 Y1,通过同源重组在ribAH结构基因中引入了两个连续的终止密码子,得到ribAH基因缺陷株B. subtilis 24 Y3。
参考来源 - 产核黄素枯草芽孢杆菌ribAH基因在ccpA基因位点的表达·2,447,543篇论文数据,部分数据来源于NoteExpress
用单链构象多态性对扩增片段进行点突变检测。
The point mutations in these fragments were detected by SSCP.
运用核酸酶保护试验观察rb基因的点突变现象。
Applying RNase Protection assay, the point mutation in the RB gene was observed.
错义突变引起蛋白质中一个氨基酸变化的点突变。
Missense mutation a point mutation that causes a change in one amino acid of a protein.
And one bit of evidence that these genes are implicated is that some unfortunate people have point mutations in these genes.
证明这些基因起作用的一些证据,是这些基因会在某些不幸的人身上,发生点突变
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