方法用淋巴细胞分层法和低渗溶血法提取PBMC。
Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.
改良酶消化法和刮取法收集人输卵管上皮细胞,采用溶血法及差速贴壁法纯化上皮细胞。
Epithelial cells were collected with a modified method which included enzymatic method and scraping, and then the collected cells were purified by hemolysis method and differential adhesion method.
目的探讨不同静脉采血方法对血液标本溶血现象的影响,并总结真空采血法的护理要点及注意事项。
Objective To explore the impact of different methods in blood drawing on sample hemolysis, and to summarize the nursing points of vacuum blood drawing.
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