采用杯碟法和纸层析的方法,测定了榆耳发酵液对九种细菌的抑制作用。
The inhibitory effects of the fermentation broth of Gloeostereum incarnatum have been determined by using the cup plate method and paper chromatography.
包涵体蛋白经含8m尿素的结合缓冲液溶解后,用金属亲和层析法进行纯化,获得了高纯度的目的蛋白。
After dissolution of inclusion bodies in binding buffer containing 8m Urea, the fusion protein was purified with metal affinity chromatography column, high purity protein was achieved.
酶促反应后的产物经薄层层析、旋光度法和高效液相色谱鉴定为L -半胱氨酸。
The product was identified to be L-Cysteine based on thin layer chromatography, optical rotation and HPLC studies.
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