方法采用甲醇索氏提取，中性氧化铝柱层析分离纯化、高效液相色谱法测定连翘中连翘苷含量。
METHODS The content of phillyrin in forsythia was determinated by using Soxhlet extractor with methanol, separation and purification with the neutral aluminum oxide column and HPLC method.
样本用丙酮提取，加二氯甲烷萃取，通过中性氧化铝和活性炭混合柱层析净化，然后上气相色谱用NPD检测。
The procedure was described, including extraction by acetone and dichloromethane, cleaning up by passing through a column made up of neutral alumina and active carbon, determination by GC with NPD.
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