用随机引物pcr检测模板dna的质量,比较了不同提取缓冲液、不同温度及不同处理时间对模板dna质量的影响。
The effect of different extraction buffers, different temperatures and different treatment times on the quality of DNA were compared by examining the DNA with random-primer PCR.
方法:比较以往报道的各种低分子量蛋白的电泳分离方法,对电泳缓冲液系统、分离胶和浓缩胶的浓度、电泳程序等进行了优化。
Methods: Based on several reported SDS-PAGE systems for low molecular weight protein, the buffer systems, concentrations of separating gel and stacking gel, electrophoresis procedures were optimized.
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