标志酶指细胞中某细胞器或亚细胞结构所特有的酶,根据此酶的特异反应,可以对亚细胞结构进行定位或定性。
电镜和标志酶活性鉴定结果表明,本法能够制备具有一定产率和纯度的肝细胞质膜样品。
The plasma membrane has been identified by the electron microscopy and plasma membrane marker enzymes.
细胞色素氧化酶是线粒体电子传递呼吸链上的标志酶,对氧有高度亲和力,能够使呼吸链以最大速度发挥作用。
Cytochrome oxidase is the marking enzyme on electron transporting respiratory chain of mitochondria, with high affinity on oxygen and can make the respiratory chain react with maximal velocity.
结果:通过标志酶活力的监测,梯度离心后线粒体纯提高近12倍,线粒体蛋白质在双向凝胶电泳中得到很好的显现。
Results: the increase in purity of mitochondria was found to be 12 times by density gradient centrifugation. Mitochondrial proteins were displayed well in the 2 -...
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