柱体积是指凝胶装柱后,从柱的底板到凝胶沉积表面的体积。
分离程序:由仪器自带的32 Karat 软件控制自动运行,首先以30 倍柱体积(Column Volume,CV)的SB 平衡柱子,然后由进样器进样2 ml 样品,蛋白上样量为4 mg,用SB 洗脱20 min,再...
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根据点火过程中压强-时间和压强-体积模量变化规律,得出了药柱体积模量随时间的变化规律。
Based on the change laws of pressure-time and pressure-bulk modulus during ignition, the chang law of bulk modulus of grain with time was obtained.
结论在明胶亲和柱体积不变的条件下,制备纤维蛋白结合素的量与血浆加样量、血浆在柱内停留时间密切相关。
CONCLUSION: in a given column volume of gelatin, the quantity of purified fibronectin was closely related with the plasma retention time in column and the total loading amount of plasma.
我知道通常的规则是需要约10个柱体积的流动相来重新平衡柱子,但是我必须要验证给别人看这些重新平衡条件是充分的。
I know the general rule that approximately 10 column volumes of mobile phase is necessary for reequilibration, but I must demonstrate and validate that the reequilibration conditions are sufficient.
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