在高倍倒置显微镜下,利用显微操作器(Micromanipulator),控制显微注射针在显微镜视野内移动的机械装置,用来进行细胞或早期胚胎操作的一种方法。
misc. micro-injection
The influence that the number of embryo and different cell stages acted on lambing rate was observed in the process of microjection.
3、在显微注射时,观察了胚胎移植数及不同细胞期对产仔率的影响。
参考来源 - 向小鼠导入αTo facilitate the further functional studies of NR5A2, we plan to establish a nr5α2 transgenic mouse by using pronucleus microinjection.
为进一步深入研究NR5A2的生理功能,本研究通过原核显微注射法构建nr5a2转基因小鼠。
参考来源 - 人核受体nr5a2(hblf)转基因小鼠的建立及其分析After transfection or microinjection,the fusion protein could be expressed efficiently and localized accurately both in Hela cells and bovine oocytes. This greatly facilitates the further study of P21,especially its role on oocytes maturation and early embryo development.
p21-venus cRNA显微注射牛卵后其融合蛋白也能实现准确定位,为研究P21在卵母细胞成熟以及胚胎发育过程中的作用奠定了基础。
参考来源 - p21基因在牛卵成熟中的表达检测及其真核表达载体构建The design method of the micro-injection is proposed based on the converse piezoelectric effects,The design of micro-injection device structure is completed.
提出了利用压电陶瓷的逆压电特性进行显微注射的设计方法,完成了显微注射仪的结构设计。
参考来源 - 压电驱动下显微注射仪的设计·2,447,543篇论文数据,部分数据来源于NoteExpress
本论文是上述微流体数字化技术在细胞显微注射技术上的实验研究。
In this paper, the applied research of cell microinjection is based on the micro-fluidic digital technology above.
根据三次显微注射的结果,注射菌液后的赤眼蜂的成活率平均值仅为2.1%。
According to the results of three generations of microinjection, the average of survival rate is only to 2.1%.
该技术主要包括:目的基因和靶细胞的制备、样品的灌注以及对细胞的显微注射。
The main procedures were: to prepare the target genes and cells, to fill the sample into the glass capillary injector and to proceed microinjection.
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