结果显微分离后弹力膜及内皮细胞层联合延迟消化培养法，体外培养牛角膜内皮细胞成功率高。
Results in the culture of BCECs in vitro, the methods of separating posterior elastic layer and endothelium of cornea under microscope, combined with delay digestion proved to succeed easily.
解决了激光显微分离系统染色体样本制备的难题，使杨树花粉母细胞减数分裂染色体激光显微分离成为可能。
Some results as follows, 1. Chromosome preparation for laser micro-dissection system was solved, which indicated that laser micro-dissection of PMCs meiotic chromosomes in Populus was feasible.
材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
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