昆虫杆状病毒表达系统 Baculovirus Expression Vector System
目的建立昆虫杆状病毒表达系统表达人II型CD 74胞外端片段的技术平台。
Objective to establish an expression system of human II CD74 extracellular fragment by insect baculovirus vector expression system in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO . 7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
该病毒作为载体，应用在杆状病毒-昆虫真核生物表达系统。
It is applied as a carrier in expression system of granulosis virus insect eucaryotic biology.
应用推荐