...培养基又广泛的应用前景,许多利用基因工程技术重组的蛋白质最终要应用于人体,如果再 一、无蛋白培养基(protein free midium,PFM):即不含有动物蛋白的培养基。无血清培养基仍含有较多的动物蛋白,如胰岛素,转铁蛋白、牛血清白蛋白等。
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结论无蛋白培养基可完全取代常规的有血清培养基,用于生产高滴度、高纯度单克隆抗体。
Conclusions CD medium is able to replace serum containing medium and very suitable for production of high quality McAb.
在培养后期换用无血清培养基培养,细胞生长及蛋白表达水平正常。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
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