The first step in the process is to take our plasmid which we've selected, and to insert the gene that we want into it.

这个过程的第一步,是取得我们选择的质粒,把我们需要的基因插入到里面

耶鲁公开课 - 生物医学工程探索课程节选

For a minute just assume that we can do this and I'm going to show you how to do it on the next slide.

我们暂时先假设基因已经插入到了质粒里,我会在下一张幻灯片告诉你如何做到

耶鲁公开课 - 生物医学工程探索课程节选

The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选