荧光相图法研究牛血清白蛋白变性和复性过程，捕捉部分折叠中间态。
The unfolding and refolding of bovine serum albumins and the existence of intermediates were studied by fluorescence phase diagram.
模拟结果显示：模型蛋白分子的某些折叠中间态会陷入局部能量最低状态而无法完成折叠；
The thermodynamic feasible status of the model protein, as described by HP model, and its folding intermediates were captured.
实验结果表明荧光相图法可以检测蛋白质去折叠的中间态。
The experimental results show that the "phase diagram" method of fluorescence can be used to detect unfolding intermediates of proteins.
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