采用单价和多价抗血清建立了全菌ELISA方法，用于95株尿源性大肠杆菌的研究，并与血凝试验进行比较。
The whole-bacterium ELISA was carried out with the single or multiple valence antisera and compared with the hemagglutination test for 95 strains of E. coli isolated from urine.
通过条件试验，确定了所获抗血清和包被抗原的最佳工作浓度分别为1：6000和1：25600。
The best concentration of the antiserum and the coated antigen were fixed on 1:6000 and 1:25600 by tests on condition, respectively.
利用免疫电泳、向琼脂扩散试验分析和测定抗血清的效价。
The titer of antiserum was detected by immunoelectrophoresis and double immunodiffusion.
应用推荐