再将RB肿瘤抗原致敏树突状细胞,与自体淋巴细胞共孵育后,能诱导出特异性杀伤肿瘤细胞的CTL。
The dendritic cells were induced by RB tumor antigen and then it cooperated with RB tumor lysates in vitro to induce antigen specific CTL which can kill the SO-RB50 target cells specifically.
目的研究抗原冲击致敏的脐血树突状细胞体外对肿瘤细胞的杀伤作用。
Objective To investigate the lethal effect of dendritic cell derived from cord blood sensitized by antigen impact on tumor cell in vitro.
方法:采用脐带血来源的单核细胞制备树突状细胞,体外经s - 180细胞肿瘤相关抗原(TAA)致敏后免疫小鼠,一周后接种s - 180细胞株。
Methods: DCs were derived from cord blood monocytes, then were pulsed by tumor associated antigen (TAA) extracted from human stomach cancer S-180 cell line.
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