当发酵液中乙醇浓度为50%、70%时,分别得到两种水溶性很好的多糖。
When alcohol density of fermentation supernatant was 50% and 70%, two kinds of water-solubility polysaccharide can be acquired.
再对复筛得到的菌株进行摇瓶培养,用纤维蛋白平板法测定并比较不同菌株发酵液的纤溶活性。
The fibrinolysis activity of the fermented fluid was determined and analyzed by the thrombolysis experiment in vitro.
通过对发酵液进行溶媒抽提、柱层析、重结晶,得到纯品,收率达到63.5%,纯度96.3%。
By solvent extraction, column chromatography, and crystallization, fexofenadine (96.3% purity) was obtained with yield 63.5%.
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