Microsoft Word - 03刘静.doc 关键词： 蛋白质组学；小麦叶片；二维液相色谱；纳升级液相色谱串联质谱 [gap=2463]Key words: proteomics; wheat leaf; 2D-LC; Nano LC-MS/MS
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The two sample preparation methods provided here which can sepatate mssive protein spots and be comparable with mass spectrum could be used in wheat leaf proteome analysis in future.
本文提供的两种小麦叶片样品制备方法，可分离检测到较多的小麦叶片蛋白点，并与质谱兼容性好，有助于小麦叶片蛋白质组研究的开展。
参考来源 - 适用于小麦叶片蛋白质组分析的双向电泳技术体系的建立·2,447,543篇论文数据，部分数据来源于NoteExpress
用差速离心法分离绿色小麦叶片线粒体。
Wheat leaf mitochondria are isolated with differential centrifugation.
水分亏缺胁迫导致小麦叶片内源多胺累积。
The water deficit stress resulted in the accumulation of endogenous polyamines in wheat leaves.
并讨论了活性氧代谢在小麦叶片衰老过程中的可能作用。
The potential action of active oxygen metabolism in wheat leaf senescence process was discussed.
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