基础培养基添加血清、抗生素等物质后,叫完全培养基,也叫(血清)细胞培养基。基础培养基只能维持细胞生存,要想使细胞生长和繁殖,还需添加天然培养基,常用的是牛血清,因为牛血清中含有促细胞增殖的各种生长因子和其他多种有利于细胞生存的物质。此外为防止污染,培养液中尚需加一定量的抗生素。完全培养基根据添加血清量的多少,可分为细胞生长培养基和细胞维持培养基,用于不同细胞和不同研究。
实验结果表明,MEM-FA中培养的细胞染色体自发畸变率显著高于完全培养基(MEM)。
The chromosome aberration frequency was increased when lymphocytes were cultured in medium deficient in folate and thymidine (MEM-FA).
内皮细胞培养基是专门为正常人类微血管内皮细胞体外培养设计的最适于其生长的完全培养基。
Smooth muscle cell medium (SMCM) is a complete medium designed for optimal growth of normal human smooth muscle cells in vitro.
方法采用正交试验设计和完全随机实验设计筛选罗汉果脱毒苗茎段增殖培养基。
Methods the selection of the fitting shoot segment multiplying culture media was studied by orthogonal test design and completely random experimental design.
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