本文的主要研究目标是基于比较基因组学方法和PCR技术,获得猪BMP15基因的编码区序列(coding sequence,CDS),并建立适宜的多态性检测方法。
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基因的完整编码区序列 coding sequence ; CDS
本研究扩增得到的基因片段与报道的猪ghrh基因编码区序列完全一致。
Homogenious analysis showed that it was consistent with the reported porcine GHRH gene sequence.
经d NA序列分析表明所获基因含有全部前导序列,其成熟蛋白编码部分与从第一骨架区引物所克隆的序列相符。
DNA sequences analysis indicated that the cloned genes included the whole leader sequences and the mature Ig protein encoding regions.
在HCV的编码基因中,核心区(C区)序列具有高度遗传保守性,c基因表达产物具有良好的抗原稳定性。
Among HCV encoding gene, core region shows greater sequence conservation. Core gene expression has favorable antigen stability.
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