口蹄疫是猪、牛、羊等主要家畜和其它家养、野生偶蹄动物共患的一种急性、热性、高度接触性传染病，易感动物达70多种。临床特征是在口腔黏膜、蹄部和乳房皮肤发生水疱性疹。预防口蹄疫的疫苗，由在幼仓鼠的肾细胞中生长的病毒失活制取。当前口蹄疫苗存在的问题是疫苗缺乏稳定性，因此用分子生物学技术生产更稳定、安全的疫苗是很有吸引力的。
目的为了克服灭活口蹄疫病毒疫苗可能存在的传播病毒的潜在危险，构建一种能预防O型口蹄疫病毒感染的VP1表位重组蛋白疫苗。
Objective to developed a recombinant protein vaccine with epitopes on VP1 protein for prevention of foot-and-mouth disease virus (FMDV) spreading by inactive-FMD vaccine.
研究分析了O型口蹄疫病毒(FMDV)结构蛋白vp1与当前猪f MDV疫苗血清的免疫反应性。
In this study, the immunoreactivity of structural protein VP1 of foot-and-mouth disease virus (FMDV) type o with sera from swine vaccinated against FMDV was analyzed.
能与口蹄疫病毒感染血清发生特异性反应，而不能与疫苗免疫动物血清发生反应；
The purified protein could specificially react with FMDV infection antibodies in Western blotting assay, but no reaction with the immune antibodies induced with vaccine.
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