结论在角膜碱烧伤后的损伤机制中有变性抗原与抗体的病理性免疫反应参与，并持续较长时间存在。
Conclusion in the mechanism of the alkali burn this kind of pathological immunoreaction about denatured antigen and its specific antibody plays important operation, and exists for long time.
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理，以电泳切胶回收所获得的纯化产物作为抗原，免疫小鼠制备抗重组蛋白血清。
The recombination fusion protein was purified by lysis, washing, denaturation and renaturation. The specific band of expression was excised from the gel and used to immunize mice .
目的了解角膜碱、酸、热烧伤后有无新的角膜变性蛋白产生并检测其抗原性。
Objective To explore the change of corneal protein and determine if there is any new denatured protein and detect their antigenicity following alkali burn, acid burn and thermal burn.
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