本研究克隆了苹果NAD-SDH 基因的部分cDNA 序列，构建了其反义表达载体，并正在通过农杆菌浸染法将该基因反义转化至蔷薇科植物中。

In this dissertation, a partial fragment of NAD-SDH from apple fruit was cloned, the NAD-SDH antisense expression plasmid was constructed, and the antisense expression has been doing;

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目的构建反义MBD1基因片段真核表达载体，为研究MBD1基因功能提供工具。

Objective to construct eukaryotic expression vector of antisense MBD1 gene fragment and to provide a tool for studying MBD1 gene function.

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目的：构建CD 147反义rna表达质粒载体，探索治疗骨肉瘤侵袭和转移的新方法。

AIM: to construct eukaryotic antisense RNA expression vector of CD147 and probe into a new method to treat invasion and transfer of osteosarcoma.

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