The oocyte activation is one of the key step of nuclear transfer (NT) in mammal, and the efficiency of oocytes activation affects the efficiency of NT directly.
成熟卵母细胞移核后的激活是核移植的关键步骤之一，卵母细胞激活率的高低直接影响核移植的效率。 当前核移植效率低下，其原因之一就是受体卵母细胞未被充分激活。
参考来源 - 不同激活方法对猪卵丘细胞核移植效果的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
CB能抑制卵母细胞激活后第二极体排出，从而提高原核的形成率。
CB can increase the two pronuclei formation rate of oocytes by inhibiting the extrusion of second polar body.
综合上述结果可以看出，6 - DMAP对小鼠卵母细胞的激活作用时间窗口具有卵龄依赖性但与起始刺激后的时间关系不大。
From the above results, we concluded that the temporal window for action of 6-dmap on the oocytes depended on the egg age of oocytes but independent of the starting time of the treatment with ethanol.
结论优化的电融合参数是决定供体核-卵母细胞复合体融合与重组胚激活的关键因素。
Conclusion Optimal parameters for electrofusion are crucial for the fusion of donor-recipient pairs and the activation of the reconstructed embryo.
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