目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合，对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。
The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).
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