利用农杆菌注射法对草莓果实细胞进行了转化研究。
Fruit cell transformation of strawberry was carried out with injection of Agrobacterium.
以GUS基因作为报告基因,利用农杆菌真空渗透法,采取正交试验设计对莴苣LactucasativaL。
Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce Lactuca sativa l.
其中以开花初期的植株,利用胭脂碱型农杆菌介导(如c58),真空渗入5分钟的处理方法转化效率最高。
It was concluded that using the nopaline type of Agrobacterium (e. g. C58), 5min vacuum infiltration, and using the plants with just several blooming flowers would give the best results.
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