当国科会有一个小时可以简要克林顿、湖泊有必要切割时间50分钟10分钟我,放弃对国内的员工。
When the NSC had an hour to brief Clinton, Lake made a point of cutting the time to 50 minutes, ceding 10 precious minutes to the domestic staff.
而这么做我要切割更多的组织。
因为她认为,‘天啊,如果我不带她去做切割,她就没法嫁人了。’
Because she feels, 'Oh my god, my daughter won't get a husband if I don't cut her.
If I cut up just DNA from the chromosome, what's called genomic DNA, then I'm going to have both exons and introns within the fragments that I create.
如果我切割的恰好是染色体上的,基因组DNA,那我切成的片段上,就会同时有外显子和内含子
If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.
如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端
If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.
而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对
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