材料和方法：采用酶辅助的显微分离法分离新生牛眼ipe细胞，酶消化法分离角膜内皮细胞，进行体外细胞培养。
Material and Methods: From bovine eyes IPE cells were isolated with enzyme-assisted microdissection, corneal endothelium cells with enzyme digestion and then were cultured in vitro.
结果显微分离后弹力膜及内皮细胞层联合延迟消化培养法，体外培养牛角膜内皮细胞成功率高。
Results in the culture of BCECs in vitro, the methods of separating posterior elastic layer and endothelium of cornea under microscope, combined with delay digestion proved to succeed easily.
用HSV - 1的ACV耐药株感染兔角膜造模，分别用抗生素17997或ACV局部给药治疗，以局部病损及病毒分离判断疗效。
The efficacy of 17997 in treatment of ACV resistant HSV 1 induced rabbit keratitis was evaluated by lesion score and virus isolation.
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