避免这一问题的策略: (1)采用可诱导启动子; (2)构建分泌载体(secretion vector)。 除去过度表达的蛋白增加细胞的稳定性; 如表达的蛋白有毒性,分泌对细胞的存活 有利; 分泌有利于蛋白纯化。
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将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
目的构建结核杆菌分泌蛋白mpt53原核表达载体并进行表达和纯化。
Objective to construct a expression vector of MPT53 of mycobacterium tuberculosis and identify and purify the protein in the e.
外源淀粉水解酶能在酵母中高效表达并分泌,其主要因素在于:在构建载体时组入强启动子;
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
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